Turkish Journal of
Endocrinology and Metabolism
Original Article

Expression Level of Circulating miR-93 in Serum of Patients with Diabetic Nephropathy

1.

Zanjan University of Medical Sciences, Department of Medical Genetics and Molecular Medicine, Zanjan, Iran

2.

Tehran University of Medical Sciences, Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran, Iran

3.

Tehran University of Medical Sciences, Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran, Iran

Turk J Endocrinol Metab 2018; 22: 78-84
DOI: 10.25179/tjem.2018-59661
Read: 81 Downloads: 29 Published: 01 June 2018

ABSTRACT

Objective: Diabetic nephropathy is a long-term complication of diabetes and is manifested as reduced glomerular filtration rate, increased urinary albumin excretion, and glomerular lesions. The study aim was to determine the expression level of serum cell-free mir-93 in diabetic patients with or without DN and compare that to healthy controls.

Material and Methods: In this case-control study, 61 type 2 diabetes patients (21 without diabetic nephropathy, 20 with microalbuminuria and 20 with macroalbuminuria), and 22 healthy controls were included. Cell-free microRNA was extracted from the serum of participants and real-time polymerase chain reaction was performed using SYBR Green master mix. The gene of hsa-miR-16 was used as a reference gene.

Results: Our findings revealed a significant downregulation of miR-93 expression in the serum of diabetic patients with or without nephropathy compared to the healthy individuals (p<0.005). However, there was no significant difference between the three groups of diabetic patients presenting different degrees of nephropathy.

Conclusion: Serum miR-93 is a good diagnostic marker for diabetes, but is not useful to distinguish between the diabetics with and without nephropathy.

 

 

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ISSN 1301-2193 EISSN 1308-9846