ORIGINAL ARTICLE

Serum Paraoxonase-1 Activity and Paraoxonase Q192 Gene Polymorphism in a Young, Healthy Population
Sağlıklı Genç Popülasyonda Serum Paraoksonaz-1 Aktivitesi ve Paraoksonaz Q192 Gen Polimorfizmi
Received Date : 05 Dec 2020
Accepted Date : 04 May 2021
Available Online : 04 Jun 2021
Doi: 10.25179/tjem.2020-80427 - Makale Dili: EN
Turk J Endocrinol Metab. 2021;25:193-201
Bu makale, CC BY-NC-SA altında lisanslanmış açık erişim bir makaledir.
ABSTRACT
Objective: Paraoxonase-1 (PON1) enzyme and PON Q192R gene polymorphism are associated with atherosclerosis in many middle-aged individuals and high populations at risk of cardiovascular disease. This study aimed to determine serum PON-1 and arylesterase (ARE) activities, PON Q192R polymorphism, and their association with gender, lipoprotein levels, and carotid intima-media thickness (CIMT) among young healthy individuals. Material and Methods: Four hundred fifteen healthy volunteers (F/M: 213/202, 19-25 years) were included in the study. Serum lipoprotein levels and PON1, ARE activities were measured using spectrophotometric methods. PON 192Q polymorphism was evaluated by polymerase chain reaction, and CIMT was measured using Doppler ultrasonography. Results: Serum PON1 activity and PON Q192R polymorphism frequencies were similar between men and women, while ARE activity was significantly higher in women. PON1 activity was considerably higher in RR carriers than in QQ and QR carriers among both men and women. Serum high-density lipoprotein level was lower, and CIMT was higher in men compared to women. Conclusion: PON1 enzyme activities did not differ according to gender, and ARE activity was higher in women. High PON1 activity is associated with PONQ192 RR polymorphism carriers. PON Q192 gene polymorphism could be a determinant of PON activity.
ÖZET
Amaç: Paraoksonaz-1 (PON1) aktivitesi ve Q192R gen polimorfizminin kardiyovasküler riskii yüksek ve yaşlı popülasyonlarda ateroskleroz ile ilişkisi gösterilmiştir. Bu çalışmanın amacı, sağlıklı genç popülasyonda serum PON-1 ve arilesteraz (ARE) aktivitelerini ölçmek ve bu düzeylerin cinsiyet, lipoprotein düzeyleri, PON Q192R gen polimorfizmi ve karotis intima medya kalınlığı ile (KIMK) ilişkisini değerlendirmektir. Gereç ve Yöntemler: Dört yüz on beş sağlıklı gönüllü (K/E: 213/202, minimum-maksimum 19-25 yaş) çalışmaya dâhil edildi. Serum lipoprotein düzeyleri PON-1 ve ARE aktiviteleri spektrofotometrik yöntem ile ölçüldü. PON Q192R gen polimorfizmi polimeraz zincirleme reaksiyonu, KIMK Doppler ultrasonografi ile değerlendirildi. Bulgular: Serum ARE aktivitesi kadınlarda erkeklere göre daha yüksek bulundu, ancak PON1 aktiviteleri her 2 grupta benzerdi. PON Q192R gen polimorfizm sıklıkları ve dağılımları kadın ve erkeklerde benzerdi. Tüm çalışma grubunda PON1 aktivitesi, PON Q192R RR taşıyıcılarında QQ ve QR taşıyıcılarından daha yüksek izlendi. Erkeklerde, kadın grubuna göre serum yüksek yoğunluklu lipoprotein düzeyi düşük, KIMK ölçümü yüksek tespit edildi. Sonuç: PON1 enzim aktivitesi cinsiyete göre farklılık göstermemiş olup, ARE aktivitesi kadınlarda daha yüksek izlenmiştir. PONQ192 RR polimorfizm “Yüksek PON1 aktivitesi, PONQ192 RR polimorfizm taşıyıcıları ile ilişkilidir.” PON Q192R gene polimorfizmi PON aktivitesinin belirleyicisi olabilir.
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