ORIGINAL ARTICLE
Serum Levels of 8-OHdG in Patients with Hashimoto’s Thyroiditis
Hashimoto Tiroiditi Olan Hastalarda Serum 8-OHdG Düzeyleri
Received Date : 10 Jul 2020
Accepted Date : 05 Nov 2020
Available Online : 10 Dec 2020
Fatemeh ESFAHANIAN, Seyedeh Mahdieh FOTOUK KIAIE, Roghayeh GHELICH, Zohreh JADALI*
Department of Endocrinology, Imam Khomeini General Hospital, Tehran, IRAN
*Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, IRAN
Doi: 10.25179/tjem.2020-77613 - Makale Dili: EN
Turk J Endocrinol Metab. 2020;24:321-326
ABSTRACT
Objective: A growing body of evidence shows a close relationship
between oxidative stress and autoimmune conditions
such as Hashimoto’s thyroiditis (HT). Among different
markers of oxidative damage, 8-hydroxydeoxyguanosine
(8-OHdG) is a ubiquitous marker and broadly used in research
studies. Therefore, this study aimed to measure the
level of 8-OHdG in the sera of patients with HT and healthy
control (HC) participants. Material and Methods: In this
study, patients were diagnosed with clinical (n=23) and
subclinical (n=25) hypothyroidism because of HT and compared
with 35 healthy participants. 8-OHdG was measured
using enzyme-linked immunosorbent assay in patients and
HC participants. Results: No significant difference was observed
in the mean serum 8-OHdG levels between patients
and HC participants. Conclusion: The results of this preliminary
study do not support the fact that the serum level of
8-OHdG is a biomarker of oxidative stress in patients with
HT. However, more detailed studies are needed to reveal the
exact role of 8-OHdG in this autoimmune disease of the
thyroid gland.
Keywords: 8-hydroxydeoxyguanosine; Hashimoto’s thyroiditis; oxidative stress
ÖZET
Amaç: Giderek artan sayıda kanıt, oksidatif stres ile Hashimoto
tiroiditi (HT) gibi otoimmün durumlar arasında yakın
bir ilişki olduğunu göstermektedir. Oksidatif hasarın farklı
belirteçleri arasında, 8-hidroksideoksiguanozin (8-OHdG)
yaygın bir belirteçtir ve araştırma çalışmalarında geniş ölçüde
kullanılmaktadır. Bu nedenle, bu çalışma HT hastalarının
ve sağlıklı kontrol (SK) grubunun serumlarında
8-OHdG düzeyini ölçmeyi amaçlamıştır. Gereç ve Yöntemler:
Bu çalışmada hastalara HT nedeniyle klinik (n=23)
ve subklinik (n=25) hipotiroidizm tanısı konulmuş ve 35
sağlıklı katılımcı ile karşılaştırılmıştır. 8-OHdG, hastalarda
ve SK grubunda enzime bağlı immünosorbent testi kullanılarak
ölçülmüştür. Bulgular: Hastalar ve SK grubu arasında
ortalama serum 8-OHdG düzeylerinde anlamlı bir fark izlenmemiştir.
Sonuç: Bu ön çalışmanın sonuçları, HT hastalarında
serum 8-OHdG düzeyinin oksidatif stresin bir
biyobelirteci olduğu gerçeğini desteklememektedir. Bununla
birlikte, tiroid bezinin bu otoimmün hastalığında 8-
OHdG'nin tam rolünü ortaya çıkarmak için daha detaylı çalışmalara
ihtiyaç vardır.
Anahtar Kelimeler: 8-hidroksideoksiguanozin; Hashimoto tiroiditi; oksidatif stres
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