ISSN: 1301-2193 E-ISSN: 1308-9846
  • Turkish Journal of
    Endocrinology and Metabolism
ORIGINAL ARTICLE

Serum Levels of 8-OHdG in Patients with Hashimoto’s Thyroiditis
Hashimoto Tiroiditi Olan Hastalarda Serum 8-OHdG Düzeyleri
Received Date : 10 Jul 2020
Accepted Date : 05 Nov 2020
Available Online : 10 Dec 2020
Doi: 10.25179/tjem.2020-77613 - Makale Dili: EN
Turk J Endocrinol Metab. 2020;24:321-326
Bu makale, CC BY-NC-SA altında lisanslanmış açık erişim bir makaledir.
ABSTRACT
Objective: A growing body of evidence shows a close relationship between oxidative stress and autoimmune conditions such as Hashimoto’s thyroiditis (HT). Among different markers of oxidative damage, 8-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous marker and broadly used in research studies. Therefore, this study aimed to measure the level of 8-OHdG in the sera of patients with HT and healthy control (HC) participants. Material and Methods: In this study, patients were diagnosed with clinical (n=23) and subclinical (n=25) hypothyroidism because of HT and compared with 35 healthy participants. 8-OHdG was measured using enzyme-linked immunosorbent assay in patients and HC participants. Results: No significant difference was observed in the mean serum 8-OHdG levels between patients and HC participants. Conclusion: The results of this preliminary study do not support the fact that the serum level of 8-OHdG is a biomarker of oxidative stress in patients with HT. However, more detailed studies are needed to reveal the exact role of 8-OHdG in this autoimmune disease of the thyroid gland.
ÖZET
Amaç: Giderek artan sayıda kanıt, oksidatif stres ile Hashimoto tiroiditi (HT) gibi otoimmün durumlar arasında yakın bir ilişki olduğunu göstermektedir. Oksidatif hasarın farklı belirteçleri arasında, 8-hidroksideoksiguanozin (8-OHdG) yaygın bir belirteçtir ve araştırma çalışmalarında geniş ölçüde kullanılmaktadır. Bu nedenle, bu çalışma HT hastalarının ve sağlıklı kontrol (SK) grubunun serumlarında 8-OHdG düzeyini ölçmeyi amaçlamıştır. Gereç ve Yöntemler: Bu çalışmada hastalara HT nedeniyle klinik (n=23) ve subklinik (n=25) hipotiroidizm tanısı konulmuş ve 35 sağlıklı katılımcı ile karşılaştırılmıştır. 8-OHdG, hastalarda ve SK grubunda enzime bağlı immünosorbent testi kullanılarak ölçülmüştür. Bulgular: Hastalar ve SK grubu arasında ortalama serum 8-OHdG düzeylerinde anlamlı bir fark izlenmemiştir. Sonuç: Bu ön çalışmanın sonuçları, HT hastalarında serum 8-OHdG düzeyinin oksidatif stresin bir biyobelirteci olduğu gerçeğini desteklememektedir. Bununla birlikte, tiroid bezinin bu otoimmün hastalığında 8- OHdG'nin tam rolünü ortaya çıkarmak için daha detaylı çalışmalara ihtiyaç vardır.
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