ORIGINAL ARTICLE

Genistein Supplementation Alters the Expression Levels of miR-155 and miR-181b in the Uterus of Estrogen-Deficient Rats
Genistein Takviyesi Östrojen Eksikliği Olan Sıçanların Uterusunda miR-155 ve miR-181b Ekspresyon Düzeylerini Değiştiriyor
Received Date : 13 Mar 2021
Accepted Date : 09 Nov 2021
Available Online : 29 Nov 2021
Doi: 10.25179/tjem.2021-83053 - Makale Dili: EN
Turk J Endocrinol Metab. 2021;25:379-386
Bu makale, CC BY-NC-SA altında lisanslanmış açık erişim bir makaledir.
ABSTRACT
Objective: Estrogen deficiency in menopausehas been associated with several complications like dementia, diabetes, and sexual disturbances. Estrogen modulated microRNA (miR)-155 and miR-181b are involved in various inflammatory responses. Phytoestrogens have been suggested to modulate the expression levels of uterine miRs. The present work evaluated the effect of genistein treatment on the expression levels of miR-155 and miR-181b in the uterus of ovariectomized rats. Material and Methods: Thirty female Wistar rats (180-220 g) were randomly divided into 3 groups (n=10 in each group), viz., sham: Rats that underwent laparotomy without ovariectomies, OVX: Rats that underwent bilateral ovariectomy, and OVX.G: Ovariectomized rats with 8 weeks of genistein treatment (1 mg/kg, subcutaneous, daily). Later, the uterus tissue was removed, and the expression levels of miR-155 and miR-181b were evaluated by real-time polymerase chain reaction in the experimental groups. Hematoxylin-eosin staining was used to assess histomorphological changes occurring in the uterus. Results: Genistein treatment decreased miR-155 and increased miR-181b expression levels in the uterus of estrogen-deficient rats. Moreover, genistein supplementation ameliorated histological changes in the uterus of ovariectomized rats. Conclusion: Genistein supplementation was responsible for partially ameliorating histological changes induced by estrogen deficiency and expression levels of miRNAs in the uterus of ovariectomized rats. Further investigations are warranted to investigate the inclusive effects of genistein treatment in menopausal women.
ÖZET
Amaç: Menopozdaki östrojen eksikliği; demans, diyabet ve cinsel sorunlar gibi çeşitli komplikasyonlarla ilişkilendirilmiştir. Östrojenin modüle ettiği mikroRNA (miR)-155 ve miR-181b, çeşitli inflamatuar yanıtlarda rol oynar. Fitoöstrojenlerin, uterus miR’lerinin ekspresyon seviyelerini modüle ettiği öne sürülmüştür. Bu çalışmada, genistein tedavisinin overektomize sıçanların uterusundaki miR-155 ve miR-181b ekspresyon seviyeleri üzerine etkisi değerlendirilmiştir. Gereç ve Yöntemler: Otuz dişi Wistar sıçanı (180-220 g) rastgele 3 gruba ayrıldı (her grupta n=10); sham: Overektomi yapılmadan laparotomi uygulanan sıçanlar, OVX: Bilateral overektomi yapılan sıçanlar ve OVX.G: Overektomi yapılan ve 8 haftalık genistein tedavisi (1 mg/kg, subkütan, günlük) alan sıçanlar. Daha sonra uterus dokusu çıkarılarak, deney gruplarında miR-155 ve miR-181b ekspresyon seviyeleri gerçek zamanlı polimeraz zincir reaksiyonu ile değerlendirildi. Uterusta meydana gelen histomorfolojik değişiklikleri değerlendirmek için hematoksilen-eozin boyaması kullanıldı. Bulgular: Genistein tedavisi, östrojen eksikliği olan sıçanların uterusunda miR-155 ekspresyon seviyelerini azalttı, miR-181b ekspresyon seviyelerini ise artırdı. Dahası genistein takviyesi, overektomize sıçanların uterusundaki histolojik değişiklikleri iyileştirdi. Sonuç: Genistein takviyesi, overektomize sıçanların uterusunda östrojen eksikliğinin neden olduğu histolojik değişiklikleri ve miRNA’ların ekspresyon seviyelerini kısmen iyileştirmiştir. Menopozdaki kadınlarda, genistein tedavisinin kapsayıcı etkilerini araştırmak için daha fazla araştırma yapılması gereklidir.
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